SOLIDNESS – Surveillance Of mobiLome meDiated aNtibiotic rEsiStance Spread
Mobile genetic elements (MGEs) are DNA molecules that often carry important genes for microorganism’s fitness, such as resistance and virulence genes, which may confer an adaptive advantage to recipient bacteria. Hence, they play a pivotal role in horizontal gene transfer. MGEs are one of the main players in antibiotic resistance dissemination and can be shared by different bacterial strains, species or even genera, which makes them one of the biggest concerns to healthcare stakeholders. MGEs are challenging to be characterized through sequencing, due to their chimeric, modular and repetitive nature. Our main objective is to establish a network of excellence for surveillance of MGE-mediated antibiotic resistance spread. This network will improve the access to high-quality and curated MGEs sequencing data that will be shared at the international level. It will result in the production of documents (standard operating procedures, SOPs) by the network detailing 1) harmonization of high-quality sequencing standards and protocols for MGEs detection; 2) definition of a bioinformatics workflow for MGEs sequence analysis from next-generation sequencing data; 3) definition of new sequence-based typing methods of plasmids for both Gram-positive and Gram-negative bacteria. Since the network includes a wide range of stakeholders with numerous expertise, including classical typing methods, “-omics”, bioinformatics and plasmid-detection, that will contribute to the creation of the high-quality and curated MGEs database from different sources, this proposal aims to track the evolution and spread of antimicrobial resistance and virulence in bacteria, mediated by MGEs, and in the future, find ways to prevent it.
Prof. dr. John W. Rossen
Dr. Natacha Couto
inCreasing cOmmunicatioN, awareNEss and data sharing in a global approaCh against resistance
The JPIAMR-VRI aims to establish a durable International Virtual Institute (VRI) which scope is epidemiological, diagnostic, clinical, therapeutic and translational research in AMR. The main objective of VRI will be to identify priorities on the research in the field of AMR, in a One Health approach, through the creation of a platform that will allow knowledge, resource, findings, database exchange, and connection between researches. The output of the VRI will be to provide an update vision on current and future research on AMR in Europe, and also to provide researchers, facilities and infrastructure of tools to harmonize research, to avoid duplication, and to share findings, databases and opportunities for better and efficient research studies and shared strategic priorities that take into account relevance, sustainability, engagement, innovation.
The consortium consists of 19 project members from Turkey and 13 European countries with Nicola Petrosillo MD, National Institute for Infectious Diseases, Italy as the coordinator. As a projectpartner our main task will be assessing the current research on microbiological methods for AMR detection.
Prof. dr. Nicola Petrosillo
Dr. Natacha Couto, Prof. dr. John W. Rossen